| Issue |
E3S Web Conf.
Volume 363, 2022
XV International Scientific Conference on Precision Agriculture and Agricultural Machinery Industry “State and Prospects for the Development of Agribusiness - INTERAGROMASH 2022”
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|---|---|---|
| Article Number | 03020 | |
| Number of page(s) | 6 | |
| Section | Precision Agriculture. Yield Monitoring and Estimation | |
| DOI | https://doi.org/10.1051/e3sconf/202236303020 | |
| Published online | 14 December 2022 | |
Acoustic-mechanical effect on the sperm of sturgeon fish using piezoactuators
1 Federal Research Centre the Southern Scientific Centre of the Russian Academy of Sciences, 41, Chekhov Ave., Rostov-on-Don, 344006, Russia
2 Astrakhan State Technical University, 16, Tatishchev St., Astrakhan, 414056, Russia
3 Don State Technical University, 1, Gagarin Sq., Rostov-on-Don, 344003, Russia
* Corresponding author: firsovaangelina1991@mail.ru
To improve the quality of the frozen material during cryopreservation, scientists apply various effects on cells: mechanical, chemical or physical. In this work we use acoustic-mechanical effects on cells before cryopreservation. As a result of the studies, the optimal parameters of the impact of the piezoactuator were selected to improve the quality of defrosted reproductive cells of male sturgeons. The object of research was the sperm of the Russian sturgeon. The progressive motility time of native spermatozoa posure time (0.5 min; 1 min, 1.5 min) and frequency (300 Hz, 500 Hz, 550 Hz) were used. Analysis of the motility of thawed sperm showed that the best result in terms of the percentage of sperm motility was obtained when using a frequency of 500 Hz for 1 minute (27%). At the same time, the best indicator of sperm motility time was given by using a frequency of 300 Hz for 1 minute (390 s).
© The Authors, published by EDP Sciences, 2022
This is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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