| Issue |
E3S Web Conf.
Volume 363, 2022
XV International Scientific Conference on Precision Agriculture and Agricultural Machinery Industry “State and Prospects for the Development of Agribusiness - INTERAGROMASH 2022”
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|---|---|---|
| Article Number | 03069 | |
| Number of page(s) | 9 | |
| Section | Precision Agriculture. Yield Monitoring and Estimation | |
| DOI | https://doi.org/10.1051/e3sconf/202236303069 | |
| Published online | 14 December 2022 | |
The effectiveness of using lipid- and water-soluble analogues of vitamin E as antioxidant protectors for cryopreservation of sperm of sterlet (Acipenser ruthenus) and Russian sturgeon (Acipenser gueldenstaedtii)
1 Federal research centre the Southern scientific centre of the Russian academy of sciences, Chekhov ave., 41, Rostov-on-Don, 344006, Russia
2 Astrakhan State Technical University, Tatishchev St., 16, Astrakhan, 414056, Russia
3 Don State Technical University, Gagarin sq. 1, Rostov-on-Don, 344003, Russia
* Corresponding author: kafavb@mail.ru
The aim of the research was to comparative study the effectiveness of synthetic antioxidants – lipid-soluble (butylated hydroxytoluene, BHT) and water–soluble (trolox) analogues of vitamin E for the cryopreservation of Russian sturgeon and sterlet sperm in the presence of the modified Stein’s medium. The ability of phenolic antioxidants to reduce the level of lipid peroxidation of sturgeon sperm, beneficial effect on the motility of thawed sperm has been shown. The addition of lipid- and water-soluble antioxidants to a basic cryomedim increased the fertilization rate of Russian sturgeon and sterlet sperm cells about 10% compared to the control experiment. The insignificant protective effect of these antioxidants presumably is explained by the use of a cryogenic medium with additive of toxic DMSO, therefore, it is necessary to develop a new composition of the cryo-medium with a minimum concentration of DMSO.
© The Authors, published by EDP Sciences, 2022
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