| Issue |
E3S Web of Conf.
Volume 381, 2023
International Scientific and Practical Conference “Development and Modern Problems of Aquaculture” (AQUACULTURE 2022)
|
|
|---|---|---|
| Article Number | 01074 | |
| Number of page(s) | 6 | |
| Section | Agriculture, River Ecosystems and Environment | |
| DOI | https://doi.org/10.1051/e3sconf/202338101074 | |
| Published online | 14 April 2023 | |
Application of piezoactuators in the technology of low-temperature preservation of fish reproductive cells
1 Federal Research Centre the Southern Scientific Centre of The Russian Academy of Sciences, Chekhov ave., 41, Rostov-on-Don, 344006, Russia
2 Astrakhan State Technical University, Tatishchev St., 16, Astrakhan, 414056, Russia
3 Don State Technical University, Gagarin sq. 1, Rostov-on-Don, 344003, Russia
4 FSBSI “ARC “Donskoy”, Nauchny Gorodok Str.3, Zernograd, 347740, Russia
* Corresponding author: firsovaangelina1991@mail.ru
The sperm of Russian sturgeon (Acipenser gueldenstaedtii Brandt et Ratzeburg, 1833) was the research object. Acoustic-mechanical influence on sturgeon semen improves fish-biological and reproductive indices of the seminal fluid. The most optimal indices for reproductive cells of male Russian sturgeon are 500 Hz, 90 V with the exposure duration of 1 minute. It has been shown that when a piezo-actuator with a frequency of 500 Hz and an amplitude of 90 V is applied to the sperm mixture with the cryoprotectant, ice crystals are formed later, which may influence the survival of cells after defrosting.
Key words: cryopreservation / reproductive cells of fish / sturgeon / acoustic-mechanical impact / freezing / cracking ice / microparticles
© The Authors, published by EDP Sciences, 2023
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