| Issue |
E3S Web of Conferences
Volume 1, 2013
Proceedings of the 16th International Conference on Heavy Metals in the Environment
|
|
|---|---|---|
| Article Number | 41037 | |
| Number of page(s) | 3 | |
| Section | Addendum | |
| DOI | https://doi.org/10.1051/e3sconf/20130141037 | |
| Published online | 22 July 2014 | |
Evaluation and Determination of Heavy Metals (Mercury, Lead and Cadmium) in Human Breast Milk
1 Department of Environmental Chemistry, Islamic Azad University Khorasgan (Isfahan) Branch, 8159-158 Isfahan, IRAN
2 Department of Chemistry, Islamic Azad University North Tehran Branch, Tehran, IRAN
Mercury, Lead and Cadmium were determined in 100 samples of human breast milk samples from urban and rural mothers in Isfahan (IRAN). A questionnaire about area of residence, nutrition, smoking habits, and dental fillings was filled out by the lactating mothers. The combination of nitric acid, hydrogen peroxide and perchloric acid was found to be one of the most suitable acids in wet digestion of milk. Cold vapor atomic absorption was used to determine the mercury content in milk after wet digestion. The effect of concentration of nitric acid, influence of flow rate and tin(П) chloride were investigated. The mean concentration of mercury in human breast milk samples was 0.96 ppb. Extraction of Pb and Cd were performed with ammonium pyrrolidine dithiocarbamate (APDC) to methyl isobutyl ketone (MIBK) and were determined by Flame Atomic Absorption Spectrometry. The factors influencing, the complex formation, pH, time and buffer were optimized. The mean concentration of Pb and Cd in human breast milk was 0.0147 and 0.0121 ppm, respectively. The maximum concentrations were found in breast milk of rural mothers.
Key words: Heavy metals / Mercury / Lead / Cadmium / human breast milk / Cold vapor atomic absorption / Flame Atomic Absorption Spectrometry
© Owned by the authors, published by EDP Sciences, 2014
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