E3S Web Conf.
Volume 17, 20179th Conference on Interdisciplinary Problems in Environmental Protection and Engineering EKO-DOK 2017
|Number of page(s)||8|
|Published online||24 May 2017|
Norfloxacin degradation by Bacillus subtilis strains able to produce biosurfactants on a bioreactor scale
1 Department of Environmental Microbiology, Institute for Ecology of Industrial Areas, 40-844 Kossutha 6 Katowice, Poland
2 Department of Biochemistry, Faculty of Biology and Environmental Protection, University of Silesia in Katowice, 40-032 Jagielońska 28 Katowice, Poland
3 Department of Environmental Microbiology, Institute for Ecology of Industrial Areas, Katowice, Poland
* Corresponding author: email@example.com
The discharge of antibiotics into the environment has become a major concern since this group of pharmaceuticals influence on microbial communities not only by its mode of action, but also because of the risk of a worldwide dispersal of antibiotic resistance genes (ARG). Antibiotics residues have been found in various environments such as waters, sediments, and soils. Moreover, most WWTPs are not designed to treat such kind of pollutants, which remain incompletely removed. Currently, biodegradation processes which involved bacterial strains with increased degradation capabilities is one of the most promising technique. The aim of this study was to evaluate the norfloxacin biodegradation potential of the three Bacillus subtilis strains named T-1, T’-1 and I’-1a on a bioreactor scale. The aerobic degradation was conducted in a 5-liter bioreactor on minimal salts medium in co-metabolic culture supplemented with glucose. The degradation rate of norfloxacin was determined with the HPLC technique. The surface tension was determined using ring method in order to observe the changes in biosurfactants production. Also, the biofilm formation abilities of the bacteria with two quantitative methods, crystal violet (CV) method and TTC-based test and enzymes production were evaluated.
© The Authors, published by EDP Sciences, 2017
This is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Current usage metrics show cumulative count of Article Views (full-text article views including HTML views, PDF and ePub downloads, according to the available data) and Abstracts Views on Vision4Press platform.
Data correspond to usage on the plateform after 2015. The current usage metrics is available 48-96 hours after online publication and is updated daily on week days.
Initial download of the metrics may take a while.