| Issue |
E3S Web Conf.
Volume 44, 2018
10th Conference on Interdisciplinary Problems in Environmental Protection and Engineering EKO-DOK 2018
|
|
|---|---|---|
| Article Number | 00115 | |
| Number of page(s) | 8 | |
| DOI | https://doi.org/10.1051/e3sconf/20184400115 | |
| Published online | 03 July 2018 | |
Preliminary research for identification of bacteria useful in microbially induced calcium carbonate precipitation
1
Department of Hydro–Engineering and Hydraulics, Faculty of Building Services, Hydro and Environmental Engineering, Nowowiejska 20, 00–653 Warsaw, Poland
2
Department of Biology, Faculty of Building Services, Hydro and Environmental Engineering, Nowowiejska 20, 00–653 Warsaw, Poland
* Corresponding author: katarzyna_misiolek@is.pw.edu.pl
MICP (Microbially Induced Calcite Precipitation) is a new biological method in soil stabilization. This cheap and eco-friendly technique improves strength parameters of the ground such as shear strength and decreases the permeability of gravelly and sandy soil. There are variety of microorganisms that can be used in calcite precipitation. The most popular method is precipitation of calcium carbonate by bacteria. The main purpose of the article is to present the results from Gram staining of bacteria isolated from construction sites, which is the first step of their identification. Gram’s method allows to find out which morphological groups of bacteria are adapted to conditions present in soil from construction sites and therefore are potentially able to produce calcite. The article describes the methodology of isolation, staining and determination of morphological types of bacteria.
© The Authors, published by EDP Sciences, 2018
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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