E3S Web of Conferences
Volume 44, 201810th Conference on Interdisciplinary Problems in Environmental Protection and Engineering EKO-DOK 2018
|Number of page(s)||7|
|Published online||03 July 2018|
Protozoa enumeration via microscope – some remarks on methodology
Wroclaw University of Technology, Faculty of Environmental Engineering, Wybrzeże Stanisława Wyspiańskiego 27, 50-370 Wrocław, Poland
* Corresponding author: firstname.lastname@example.org
For proper enumeration of protozoa in activated sludge good methodology is required. In this paper we present some remarks on microscopic methodology of protozoa enumeration. This remarks concern number of repetitions from one sample required to obtain good statistical results as well as influence of sample aeration on number of found protozoa. Presented data shows that at last 10 repetitions are required from each sample to obtain low average confidence interval. Lower number of repetitions leads to sharp increase in average confidence interval and loss of statistical significance while higher number does not decrease average confidence interval substantially. As measurements lasts for few hours lack of sample’s aeration during measurement leads to detection of lower by 27% number of protozoa.
© The Authors, published by EDP Sciences, 2018
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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