E3S Web Conf.
Volume 78, 20192018 International Seminar on Food Safety and Environmental Engineering (FSEE 2018)
|Number of page(s)||4|
|Section||Nutrition and Health, Medical Treatment and Biomedical Engineering|
|Published online||15 January 2019|
Effect of Wasabi Extract on Inflammatory Response of BV2 Cells Induced by Lipopolysaccharide
School of Electronic Information in Jiangsu, Nantong University, Nantong 226019, China
* Corresponding author: Qiu Yuanyuan: email@example.com
Objective: This study was to investigate the effect of wasabi extract 6-(methylsulfinyl)hexyl isothiocyanate (6-MITC) on preventing neuroinflammation. Methods: The activation of BV2 microglia cell was introduced by LPS to establish an in vitro neuroinflammatory cell model, and the influence of 6-MITC on the survival rate of BV2 cells was deceted by MTT assay and the mRNA expression level of inflammatory factors in each group were deceted by RT-qPCR. Results: 6-MITC can significantly down-regulate mRNA expression levels of the inflammatory factor interleukin-1β, IL-6, tumor necrosis factor-α (TNF-α) and nitric oxide synthase (iNOS) after activation of BV2 cells induced by LPS. Conclusion: 6-MITC can inhibit LPS-induced microglia activation and decrease inflammatory response of BV2 cells.
© The Authors, published by EDP Sciences, 2019
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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