E3S Web Conf.
Volume 100, 201911th Conference on Interdisciplinary Problems in Environmental Protection and Engineering EKO-DOK 2019
|Number of page(s)||7|
|Published online||10 June 2019|
Preliminary ecotoxicity assessment of selected flavour and fragrance compounds using Microtox assay
1 Wrocław University of Science and Technology, Faculty of Environmental Engineering, Wyb. Wyspiańskiego 27, 50-370, Wrocław, Poland
2 Wrocław University of Science and Technology, Faculty of Chemistry, Wyb. Wyspiańskiego 27, 50-370, Wrocław, Poland
3 Liquid Technologies sp. z o.o., ul. Chełmońskiego 12, 51-630, Wrocław, Poland
* Corresponding author: firstname.lastname@example.org
The bioluminescence inhibition bioassay using marine bacteria Vibrio fischeri is widely used as a tool to assess the toxicity of chemical compounds, because of the many benefits comprising cost and time effectiveness, rapidness, sensitivity, and ease of operation. In addition, the test is used for various types of organic and inorganic compounds. Due to the insolubility of organic compounds in water, a suitable solubilizer should be applied. The ecotoxicity of the solvent should be negligible in relation to marine bacteria. On account of superior human activities the synthesis of new chemical substances is inextricably linked to the emergence of chemical compounds that are a potential threat on environment. A Microtox system utilizing the 81.9% Basic Test with 14 dilutions was applied to pre-evaluate the ecotoxicity of β-cyclocitral oxime, citronellal oxime and perillaldehyde oxime. Substances solutions with an initial concentration of 0.036% in DMSO were prepared. The preliminary results show that the studied scent compounds are characterized by quite high toxicity. In order to confirm the received ecotoxicity values, it is necessary to carry out additional surveys using another range of concentrations and biotests on organisms at each trophic level.
© The Authors, published by EDP Sciences, 2019
This is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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