E3S Web Conf.
Volume 118, 20192019 4th International Conference on Advances in Energy and Environment Research (ICAEER 2019)
|Number of page(s)||8|
|Section||Energy Engineering, Materials and Technology|
|Published online||04 October 2019|
Identification and Analysis of Secondary Metabolites from CH3OH/H2O Extract of Metacordyceps Neogunnii
College of Life and Environment Science, Huangshan University, Huangshan, Anhui 245041, China
2 Analysis and Testing Center, Huangshan University, 245041, Huangshan, Anhui, China
3 School of Tourism, Huangshan University, 245041, Huangshan, Anhui, China
4 Institute of Fungus Resources, Department of Ecosystem, College of Life Science, Guizhou University, 550025, Guiyang, China
* Corresponding author: firstname.lastname@example.org
Secondary metabolites are considered to be the major compounds in Cordyceps with anti-tumor, anti-aging and immunity-enhancing effects. The molecular structures of secondary metabolites form the basis for the development and utilization of Cordyceps. Metacordyceps neogunnii is an important Cordyceps resource, but less study has been made on the molecular structure of its secondary metabolites. In this study, gas chromatography-mass spectrometry (GC/MS) and ultra performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS) were used to analyze and identify the secondary metabolites from CH3OH/H2O extract of Metacordyceps neogunnii. The results show that a total of 22 compounds were identified by GC-MS, including 2 n-alkanes, 1 isoparaffin, 1 cycloalkane, 6 olefins and 13 esters. The predominant compounds were (E)-ocimene, (E)-β-ocimene, methyl oleate, dioctyl adipate, methyl palmitate and methyl linoleate, obtained by means of GC/MS. Five distince classes of secondary metabolites were speculated: 3 polypeptides, 2 esters, 1 isoflavone, 1 isoindrone and 3 amides, from which 10 compounds were detected using UPLC-Q-TOF/MS. (R)-N-((1-(((9H-fluorene-9-yl) methoxy) carbonyl) pyrrolidine-2-yl) methyl)-N-(2-(6-benzoylamino-9H-purin-9-yl) acetyl) glycine, benzyl (5-(2-((3-(2, 3-dihydrobenzo[b] [1, 4] dioxin-6-yl)-4-carbonyl-4H-chromene-7-yl) oxo) acetylamino) amyl) carbamate, 5, 5’-((propane-2, 2-diyl (4, 1-phenylene)) bis (oxo)) bis (2-(naphthalene-1-yl) isoindoline-1, 3-dione), 1-dodecylazepine-2-one and other compounds were the first detected in Metacordyceps neogunnii.
© The Authors, published by EDP Sciences, 2019
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