E3S Web Conf.
Volume 131, 20192nd International Conference on Biofilms (ChinaBiofilms 2019)
|Number of page(s)||4|
|Published online||19 November 2019|
Induction of Adventitious buds from stem explants in Aoectochilus formosanus
Department of Resources and Environmental Sciences, College of Chemistry and Environment, Guangdong Ocean University, Zhanjiang 524088, China.
2 Department of Biotechnology, Faculty of Agricultural Science, Guangdong Ocean University, Zhanjiang 524088, China.
* Corresponding author: email@example.com.
In order to explore the optimal culture conditions for adventitious bud regeneration of stem explants in Aoectochilus formosanus, the stem segments from the sterile seedlings were used as explants, and different concentrations of diethyl aminoethyl hexanoate (DA-6), kinetin (KT), Cu2+ and glutamine (Gln) were separately added into MS medium containing 6-benzylaminopurine (6-BA) and naphthaleneacetic acid (NAA), and the induction rate and the induction multiple of adventitious buds were recorded and analyzed. The results showed that the regeneration of adventitious buds could be promoted, when 2 mg/L DA-6, 0.4 mg/L KT and 15 mg/L Gln were added in mediums. However, the effect of Cu2+ on the regeneration of adventitious buds in A. formosanus was enhanced by low concentration and suppressed by high concentration, and the best concentration of Cu2+ was 5 mg/L.
© The Authors, published by EDP Sciences, 2019
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