| Issue |
E3S Web Conf.
Volume 131, 2019
2nd International Conference on Biofilms (ChinaBiofilms 2019)
|
|
|---|---|---|
| Article Number | 01131 | |
| Number of page(s) | 4 | |
| DOI | https://doi.org/10.1051/e3sconf/201913101131 | |
| Published online | 19 November 2019 | |
Induction of Adventitious buds from stem explants in Aoectochilus formosanus
1
Department of Resources and Environmental Sciences, College of Chemistry and Environment, Guangdong Ocean University, Zhanjiang 524088, China.
2
Department of Biotechnology, Faculty of Agricultural Science, Guangdong Ocean University, Zhanjiang 524088, China.
* Corresponding author: liuying85168@126.com.
In order to explore the optimal culture conditions for adventitious bud regeneration of stem explants in Aoectochilus formosanus, the stem segments from the sterile seedlings were used as explants, and different concentrations of diethyl aminoethyl hexanoate (DA-6), kinetin (KT), Cu2+ and glutamine (Gln) were separately added into MS medium containing 6-benzylaminopurine (6-BA) and naphthaleneacetic acid (NAA), and the induction rate and the induction multiple of adventitious buds were recorded and analyzed. The results showed that the regeneration of adventitious buds could be promoted, when 2 mg/L DA-6, 0.4 mg/L KT and 15 mg/L Gln were added in mediums. However, the effect of Cu2+ on the regeneration of adventitious buds in A. formosanus was enhanced by low concentration and suppressed by high concentration, and the best concentration of Cu2+ was 5 mg/L.
© The Authors, published by EDP Sciences, 2019
This is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Current usage metrics show cumulative count of Article Views (full-text article views including HTML views, PDF and ePub downloads, according to the available data) and Abstracts Views on Vision4Press platform.
Data correspond to usage on the plateform after 2015. The current usage metrics is available 48-96 hours after online publication and is updated daily on week days.
Initial download of the metrics may take a while.