Issue |
E3S Web Conf.
Volume 245, 2021
2021 5th International Conference on Advances in Energy, Environment and Chemical Science (AEECS 2021)
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Article Number | 03024 | |
Number of page(s) | 4 | |
Section | Chemical Performance Research and Chemical Industry Technology Research and Development | |
DOI | https://doi.org/10.1051/e3sconf/202124503024 | |
Published online | 24 March 2021 |
Effects of different factors on adventitious bud induction from stem explants of Ludisia discolor
1 College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang 524088, P.R. China
2 College of Chemistry and Environment, Guangdong Ocean University, Zhanjiang 524088, P.R. China
* Corresponding author: Yingbin Xue, yingbinxue@yeah.net; Yinling Zhu, 995143667@qq.com
In this study, the stem explants of Ludisia discolor were used as experimental materials to investigate the effects of 6-BA, NAA, Cu2+ and Ag+ on the induction of adventitious bud regeneration, and to analyze the most suitable culture conditions for stem explants regeneration. The results showed that when the medium was supplemented with 1.0 mg/L 6-BA, 0.75 mg/L NAA, 0.25 mg/L CuSO4, or 6.4 mg/L AgCl, the best regeneration effects would be gained, respectively. And the adventitious bud regeneration rate reached the maximum, which were 61.67%, 83.67%, 80.95% and 87.63%, respectively. The results of this study provided a theoretical basis for tissue culture and rapid propagation of L. discolor.
© The Authors, published by EDP Sciences, 2021
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