| Issue |
E3S Web Conf.
Volume 147, 2020
The 3rd International Symposium on Marine and Fisheries Research (3rd ISMFR)
|
|
|---|---|---|
| Article Number | 01015 | |
| Number of page(s) | 4 | |
| Section | Aquaculture | |
| DOI | https://doi.org/10.1051/e3sconf/202014701015 | |
| Published online | 10 February 2020 | |
Viability of Najawa carp (Cyprinus carpio L.) sperm at 4°C temperature storage
Aquaculture Study Program, Fisheries Department, Agriculture Faculty, Universitas Gadjah Mada.
* Corresponding author: dini.sari@ugm.ac.id
The objectives of this study were to evaluate the sperm viability of Najawa carp (Cyprinus carpio L.) in cryopreservation pre-conditions at 4°C. The design used in this study was Complete Randomized Design with 4 treatments, BSS as a control, 10% DMSO, 0,2 M Sucrose, and 5% DMSO + 0,1 M Sucrose; each consist of three replications. The parameters observed were progressive motility of fresh sperm, diluted sperm before low temperature storage, and 2 hours; 3 hours; 4 hours; 5 hours; one day; one week; a month after 4°C storage. The data were analyzed by ANOVA. The data showed that there was no significant difference between treatment (P>0.05). The best viability was 40.56% of sperm motility which survive for one week, it was achieved by 5% DMSO + 0,1 M Sucrose.
© The Authors, published by EDP Sciences, 2020
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