E3S Web Conf.
Volume 151, 2020The 1st International Conference on Veterinary, Animal, and Environmental Sciences (ICVAES 2019)
|Number of page(s)||4|
|Published online||14 February 2020|
The correlation between spermatozoa morphometry and the testosterone levels in the epididymis of aceh cow
Laboratory of Anatomy, Faculty of Veterinary Medicine, Universitas Syiah Kuala, Banda Aceh 23111, Indonesia
2 Laboratory of Physiology, Faculty of Veterinary Medicine, Universitas Syiah Kuala, Banda Aceh 23111, Indonesia
3 Laboratory of Reproduction, Faculty of Veterinary Medicine, Universitas Syiah Kuala, Banda Aceh 23111, Indonesia
4 Laboratory of Histology, Faculty of Veterinary Medicine, Universitas Syiah Kuala, Banda Aceh 23111, Indonesia
* Corresponding author: firstname.lastname@example.org
The objective of this study was to determine the correlation between spermatozoa morphometry and testosterone concentration in the epididymal caput, corpus, and cauda of aceh cattle. The epididymal sperm swab slides were stained using Williams's staining to examined the morphology and morphometry of spermatozoa whereas the level of testosterone from epididymal caput, corpus, and cauda extract was measured using enzyme-linked immunosorbent assay (ELISA). The data of sperm morphology were presented descriptively whereas the data of sperm morphometry and testosterone concentration were analyzed using one way ANOVA. Pearson correlation test was conducted to know the correlation between the total length of spermatozoa (TLS) and testosterone concentration in three parts of epididymis. The result showed that the sperm morphology in caput, corpus, and cauda of epididymis was not different statistically. The TLS of aceh cattle at caput, corpus, and cauda epididymis were 69,70±0,8 µm, 70,90±0.39 µm, and 72,98±0.74 µm respectively and statistically different (p<0.05). In addition, the concentration of testosterone in caput, corpus, and cauda epididymis were 9,92±3,76 ng/g, 16.96±13,22 ng/g, and 15,63±11.80 ng/g respectively and also not different among three parts of epididymis (p>0.05). The positive correlation (r= 0.413, p= 0.416) was found between TLS and testosterone concentration at caput whereas in corpus and cauda those correlation were negative and not different (corpus: r= -0.638, p= 0.172 and cauda: r= -0.719, p= 0,108). In conclusion, the longest-sized spermatozoa found in the cauda epididymis are mature spermatozoa where the maturation process optimally takes place in caput epididymis. Testosterone found in the epididymis of aceh cow can be used as an alternative source of testosterone.
Key words: epididymis / spermatozoa / testosterone / and aceh cow
© The Authors, published by EDP Sciences, 2020
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