Identification of Active Compounds of Kepok Banana Peel and the Effect on Testosterone Concentration in Male Rats with High-Fat Diet

¹ Master of Veterinary Public Health Study Program, Faculty of Veterinary Medicine, Syiah Kuala University, Banda Aceh, Aceh, Indonesia
² Laboratory of Histology, Faculty of Veterinary Medicine, Syiah Kuala University, Banda Aceh, Aceh, Indonesia;
³ Laboratory of Anatomy, Faculty of Veterinary Medicine, Syiah Kuala University, Banda Aceh, Aceh, Indonesia;
⁴ Laboratory of Reproduction, Faculty of Veterinary Medicine, Syiah Kuala University, Banda Aceh, Aceh, Indonesia;
⁵ Laboratory of Physiology, Faculty of Veterinary Medicine, Syiah Kuala University, Banda Aceh, Indonesia

^* Corresponding author: akmal_kh@unsyiah.ac.id

Abstract

Our body needs an adequate supply of antioxidants to maintain physiological functions. Antioxidant compounds are found in natural plants, including inside of kepok bananas peel. This research was conducted to determine the potential of kepok banana peels to maintain testosterone concentration in male rats (Rattus norvegicus) with a high-fat diet. This study used 20 male rats 2.5-3 months old, which grouped into 5 groups: normal group given standard feed (K1), negative control group given high-fat diet (K2), positive control group given high-fat diet and simvastatin (K3), high-fat diet group and banana peel extract administered at 100 mg/kg BW (K4), and high-fat diet group and banana peel extract administered at 200 mg/ kg BW (K5). The blood serum was collected after 60 days of the treatment. The blood serum testosterone levels were measured by enzyme-linked immunosorbent assay (ELISA) method at a wavelength of 450 nm. The major findings of this study were that there is a significant difference between testosterone concentrations by the K4 group compared to K2 and K5 groups (p<0,05). The result suggests that the dose 100 mg/kg BW of banana peel extract has the potential to maintain the testosterone concentration in male rats with a high-fat diet.