Anti-inflammatory Activity of Ethanol Extract from Malacca Leaves (Phyllanthus emblica) in Carrageenan Induced Male Mice

¹ Clinical Laboratory Veterinary Medicine Universitas Syiah Kuala, Banda Aceh 23111, Indonesia
² Farmacology Laboratory Universitas Syiah Kuala, Banda Aceh 23111, Indonesia
³ Patology Laboratory Universitas Syiah Kuala, Banda Aceh 23111, Indonesia
⁴ Veterinary Study Program, Faculty of Veterinary Medicine Universitas Syiah Kuala, Banda Aceh 23111, Indonesia

^* Corresponding author: nuzulasmilia@unsyiah.co.id

Abstract

The purpose of this study was to determine the effect of the ethanol extract of malacca leaves (Phyllanthus emblica) to reduce carrageenan-induced edema in mice. This study used 25 mice which were divided into 5 groups with 5 mice per group. All the mice were induced inflammation using carrageenan at a dose of 1%. Mice in group 1 were not given piroxicam and malacca leaves extract. Mice in group 2 were treated with 20 mg of piroxicam suspension. Mice in groups 3, 4, and 5 were given ethanol extract of malacca leaves with doses of 100 mg/kg bb, 200 mg/kg bb, and 300 mg/kg bb, respectively. Edema volume measurements were performed on 5th day by collecting exudates using a syringe. Data were analyzed using one-way analysis of variance and continued with the Duncan test. The result showed that the average of edema volume in the negative control group was 0.09 ± 0.02 mL, while in the positive control group was 0.05 ± 0.03 mL. The average edema volume in groups 3, 4, dan 5 were 0.07 ± 0.01 mL, 0.06 ± 0.02 mL dan 0.07 ± 0.01 mL, respectively. It is concluded that the ethanol extract of malacca leaves (Phyllanthus emblica) can reduce the carrageenan-induced edema in male mice.