E3S Web Conf.
Volume 151, 2020The 1st International Conference on Veterinary, Animal, and Environmental Sciences (ICVAES 2019)
|Number of page(s)||4|
|Published online||14 February 2020|
Acute Toxicity Test of Pigeon Pea Leaves Extract (Cajanus cajan) in Rats
Department of Anatomy, Physiology, and Pharmacology, Faculty of Veterinary Medicine, IPB University (Bogor Agricultural University), IPB Darmaga Campus-Bogor 16880, Indonesia.
2 Department of Clinic, Reproduction, and Pathology, Faculty of Veterinary Medicine, IPB University (Bogor Agricultural University), IPB Darmaga Campus-Bogor 16880, Indonesia.
3 Department of Food Science and Technology, Faculty of Agricultural Technology, IPB University (Bogor Agricultural University), IPB Darmaga Campus-Bogor 16880, Indonesia.
* Corresponding author: firstname.lastname@example.org
Acute toxicity test was conducted to evaluate the safety level of pigeon pea (Cajanus cajan) leaves extract, using the Organisation for Economic Co-operation and Development (OECD 423) guideline method. The pigeon pea leaves were extracted using 96%ethanol as a solvent. A total of 9 male rats were used divided into 3 groups: 1 control group and 2 treatment groups. The rat in control group (group 1) received a single dose of distilled water while the rat in groups 2 and 3 received a single dose of pigeon pea leaves extract at doses of 300 mg/kg BW and 2000 mg/kg BW, respectively. The aquadest and extract suspension were administered orally using rat stomach tubedos. Mortality and clinical signs were examined in the first 4 hours (critical time), 24 hours, and 14 days after the treatments. The result revealed that the LD50 values of the extract was estimated at more than 5000 mg/kg BW and classified as practically nontoxic.
Key words: acute toxicity / Cajanus cajan / LD50 / rat
© The Authors, published by EDP Sciences, 2020
This is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Current usage metrics show cumulative count of Article Views (full-text article views including HTML views, PDF and ePub downloads, according to the available data) and Abstracts Views on Vision4Press platform.
Data correspond to usage on the plateform after 2015. The current usage metrics is available 48-96 hours after online publication and is updated daily on week days.
Initial download of the metrics may take a while.