E3S Web Conf.
Volume 175, 2020XIII International Scientific and Practical Conference “State and Prospects for the Development of Agribusiness – INTERAGROMASH 2020”
|Number of page(s)||7|
|Section||Livestock and Veterinary|
|Published online||29 June 2020|
The use of polyazolidineammonium and dimethyl-sulfoxide antigen Yersinia pseudotuberculosis to obtain hyperimmune serum
Saratov State Agrarian University named after N.I. Vavilova, 1, Teatralnaya Square, 410600, Saratov, Russia
* Corresponding author: email@example.com
The use of polyazolidinammonium modified with iodine hydrate ions (PAAG) as an adjuvant made it possible to obtain rabbit hyperimmune blood serums for dimethyl-sulfoxide antigen (DA) of a pseudotuberculosis microbe with generic specificity. Antibody titers in ELISA with Y. pseudotuberculosis and Y. enterocolitica cells amounted to 1:25600-1:12800, and with cells of other genera of intestinal microflora 1:100-1:400. The optimal immunizing dose for obtaining hyperimmune yersiniosis serum was a dose of 2 mg DA of Y. pseudotuberculosis per rabbit. Such a dose made it possible to obtain hyperimmune sera with a high titer of specific antibodies with a small consumption of antigen. The optimal concentration of PAAG solution for hyperimmunization of Y. pseudotuberculosis DA rabbits was 1%.
© The Authors, published by EDP Sciences, 2020
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