E3S Web Conf.
Volume 175, 2020XIII International Scientific and Practical Conference “State and Prospects for the Development of Agribusiness – INTERAGROMASH 2020”
|Number of page(s)||7|
|Section||Livestock and Veterinary|
|Published online||29 June 2020|
Pathomorphological changes in the testes of males with babesiosis
Stavropol State Agrarian University, 12, Zootekhnicheskiy per., 355017, Stavropol, Russia
* Corresponding author: firstname.lastname@example.org
The aim of the work was to study pathomorphological changes in the testes in males with babesiosis. Diagnosis of babesiosis in sick males was performed based on the detection of typical clinical signs and blood smears. Blood smears were fixed in methanol and stained according to the Romanovsky-Giemsa method with hematoxylin and eosin. For histological studies, pieces of testicles were selected, which were fixed in an 8% neutral aqueous formalin solution. The prepared material was poured into paraffin according to the conventional method. Histological sections were stained with hematoxylin and eosin using the method of Van Gieson and Mallory. Pathomorphological examination of stained histological sections revealed changes characteristic of parenchymal orchitis. The spermatogenic epithelium of the convoluted seminal tubules was drained down in all fields of vision. The lumen of the tubules was filled with protein detritus, exfoliated cells of the spermatogenic epithelium and macrophages. Part of the Sertoli cells was in a state of vacuolar degeneration. Focal clusters of lymphoid-macrophage infiltrates were visible between the convoluted seminal tubules, especially multiple ones around the blood vessels. Endocrine cells were subjected to atrophy due to compression by cell infiltrates and connective tissue growths.
© The Authors, published by EDP Sciences, 2020
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