E3S Web Conf.
Volume 203, 2020Ecological and Biological Well-Being of Flora and Fauna (EBWFF-2020)
|Number of page(s)||9|
|Section||Advances in Crop Production|
|Published online||05 November 2020|
Gmbak1 and gmfls2, two genes response to bacterials disease of soybean
Northeast Agricultural University, Harbin China.
* Corresponding author: firstname.lastname@example.org
Soybean bacterial disease is a worldwide disease that can cause yield and quality decline. Although the recognition of soybean bacterial diseases has been reported in detail, but for the identification of resistant and susceptible cultivars, different researchers have different methods and standards. To detect the pathogenic effect of bacterial diseases, we first separated bacterial strains from soybean field. Finally, eight strains of soybean bacterial diseases were isolated and identified. By the screening the sequence of the bacterial 16S rDNA, seven of eight strains were identified belong to Pseudomonas, another strain belong to Xanthomonas. In order to study the relevant pathogen of soybean defense response, we used the isolated pathogen strain to inoculate the resistant cultivar Hefeng35 and sensitive cultivar Suinong14. Then the genes might involve in regulate soybean resistant to bacterial disease were analyzed, including GmBAK1 and GmFLS2. FLS2 homolog gene were Gm05g25830, Gm08g08810; BAK1 homolog gene were Gm15g05730, Gm08g07930, Gm10g36280, Gm05g24790, Gm20g31320, Gm05g24770, Gm08g19270, and Gm02g08360. By the qRT-PCR analysis we detected the gene expression pattern response to pathogen, we found the expression of Gm05g24770, Gm05g24790, and Glyma05g25830 showed significant different between Suinong14 and Hefeng35. We illustrated the GmBAK1 and GmFLS2 transcription level against bacterial speck disease pathogen infection and revealed preliminary the major gene, providing a reference direction for further research.
© The Authors, published by EDP Sciences, 2020
This is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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