E3S Web Conf.
Volume 233, 20212020 2nd International Academic Exchange Conference on Science and Technology Innovation (IAECST 2020)
|Number of page(s)||4|
|Section||BFS2020-Biotechnology and Food Science|
|Published online||27 January 2021|
Protective Effects of Flavonoid and Polyphenol from Lotus Leaf on Lung Damage Induced by Inhalation of N2O4 in mice
1 Hubei University of Technology, Hubei Key Laboratory of Industrial Microbiology. KeyLaboratory of Fermentation Engineering (Ministry of Education), Wuhan, 430068, China.
2 National “111” Center for Cellular Regulation and Molecular Pharmaceutics, Hubei Research Center of Food Fermentation Engineering and Technology, Wuhan, 430068, China.
3 Function Food key Laboratory of Hubei province Hubei Uinversity of Chinese Medicine Wuhan, 430065, China.
To study the protective effects of flavonoid and polyphenol (FP) from lotus leaf on the damage induced by N2O4 in mice. Constructing an animal damage model through exposing mice to 45μl N2O4 in a 120 L sealed cabinet for 30 minutes. ICR male mice were randomly divided into normal group, physiological saline+N2O4 group,1.25g/kgFP +N2O4 group, 2.50g/kgFP + N2O4 group, 3.75g/kg FP +N2O4 group. 1.25, 2.50, 3.75 g/kg FP were orally administered to mice respectively for 5 days, equal volume physiological saline for normal group and physiological saline+N2O4 group. Then, the three FP groups and the physiological saline+N2O4 group were exposed to N2O4 in the cabinet on 5th day. In an hour after N2O4 exposure, killing the mice by dislocation to measure the SOD, GSH-Px activity and MDA content in lung tissue, checking pathology change in lung tissue slice. Results were shown: the 1.25, 2.50g/kg FP increased SOD (U/mg pro) by 30.3% and 24.4%, GSH-Px (U/mg pro) by 77.4% and 60.9%, respectively. Pathological observation of lung tissue showed that three FP groups had lighter damage than the physiological saline+N2O4 group. So, FP has significant protective effects on damage caused by N2O4 in mice.
© The Authors, published by EDP Sciences 2021
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