| Issue |
E3S Web Conf.
Volume 245, 2021
2021 5th International Conference on Advances in Energy, Environment and Chemical Science (AEECS 2021)
|
|
|---|---|---|
| Article Number | 03006 | |
| Number of page(s) | 4 | |
| Section | Chemical Performance Research and Chemical Industry Technology Research and Development | |
| DOI | https://doi.org/10.1051/e3sconf/202124503006 | |
| Published online | 24 March 2021 | |
Synthesis of Mesoporous Silica Nanowires and Their Application in Enzyme Immobilization
School of Biological Engineering, Tianjin Modern Vocational Technology College, Tianjin, 300350, China
* Corresponding author: wangp20@yeah.net (P. W.)
Hydrophobic mesoporous silica nanowires were synthesis and then employed as support for immobilization of lipase from Candida antarctica via covalent bonding (CALB@MSW). The parameters were optimized and the optimum conditions were as follows: GA concentration 5.5 wt.%, activation time 60 min and CALB concentration 4 mg/mL. Under these conditions, the protein loading and specific activity of CALB@MSW were 138.3 mg/gsupport and 41.1 U/mgsupport, respectively. Compared with free CALB, CALB@MSW showed better thermal stability and pH stability. The maximum yield of biodiesel catalytic by CALB@MSW was 93.4 %. After reused 8 times, CALB@MSW still remained 95.75 % initial activity showing better stability than free CALB.
© The Authors, published by EDP Sciences, 2021
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