E3S Web Conf.
Volume 251, 20212021 International Conference on Tourism, Economy and Environmental Sustainability (TEES 2021)
|Number of page(s)||4|
|Section||Environmental Ecological Analysis and Sustainable Development Research|
|Published online||15 April 2021|
Analysis of citric acid and D-isoascorbic acid in beverages by High performance liquid chromatography
1 Chongqing Collaborative Innovation Center for Functional Food, Chongqing University of Education, Chongqing 400067, China
2 College of Biological and Chemical Engineering, Chongqing University of Education, Chongqing 400067, China
* Corresponding author: email@example.com
In this study, high performance liquid chromatography (HPLC) was used to analysis the content of citric acid and D-isoascorbic acid in beverages. The samples were separated by C18 chromatography column, 0.1 % phosphoric acid solution was used as the mobile phase with the flow rate of 0.5 mL/min, the column temperature was 35 °C, and the detection wavelength was 210 nm. The results showed that the content of citric acid and D-isoascorbic acid has a good linear correlation (r>0.99) within the range of 50 μg to 200 μg. The selectivity, recovery and precision of citric acid and D-isoascorbic acid were suitable. Meanwhile, this method could be used to detect the content of citric acid and D-isoascorbic acid in beverages.
© The Authors, published by EDP Sciences, 2021
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