| Issue |
E3S Web Conf.
Volume 251, 2021
2021 International Conference on Tourism, Economy and Environmental Sustainability (TEES 2021)
|
|
|---|---|---|
| Article Number | 02056 | |
| Number of page(s) | 4 | |
| Section | Environmental Ecological Analysis and Sustainable Development Research | |
| DOI | https://doi.org/10.1051/e3sconf/202125102056 | |
| Published online | 15 April 2021 | |
High PGL productivity in Bacillus subtilis by optimizing the SD sequence
Qilu institute of technology, 250200 Jinan, China
* Corresponding author: 593183586@qq.com
Alkaline polygalacturonate lyase (PGL, EC 4.2.2.2) can catalyze the cleavage of a-1, 4-glycosidic bonds of polygalacturonate by a trans-elimination reaction and generate an unsaturated oligogalacturonates. As the critical enzyme of many environmental friendly processes, alkaline PGL has been widely used in many fields including paper, textile and beverage industries. At present, Bacillus subtilis is an ideal strain for producing PGL, but the yield is too low for industrial production. In this study, the effect of different SD sequences on the production of PGL was comparatively investigated, and the strong SD sequence (AGAGAACAAGGAGGG G) directed efficient PGL secretory expression and increased PGL yield to 264.5 U·mL-1 with a high productivity. As a result, the PGL yield in B. subtilis was effecively increased and laid the solid foundation for PGL industrial production.
© The Authors, published by EDP Sciences, 2021
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