E3S Web Conf.
Volume 251, 20212021 International Conference on Tourism, Economy and Environmental Sustainability (TEES 2021)
|Number of page(s)||4|
|Section||Environmental Ecological Analysis and Sustainable Development Research|
|Published online||15 April 2021|
Application of Different Methods to Determine Urease Activity in Enzyme Engineering Experiment and Production
1 College of Chemical and Biological Engineering, QILU Institute of Technology, Jinan, Shandong 250200.
2 College of Agriculture, Ludong University, Yantai, Shandong, 264001
3 College of Life Sciences, Ludong University, Yantai, Shandong, 264001
The determination of urease activity is one of the important projects in 《Enzyme engineering experiment》 and production. In the paper, the urease activity of soybean was determined by pH increment method, titration method and Nash reagent method, and the three methods were compared. The results showed that the urease activity of the same sample measured by pH increment method, titration method and Nash reagent method was not equal in value and could not be used mutually, and the urease activity of the same sample measured by Nash reagent method was the highest. The same experimental project adopts a variety of methods to improve students ’ interest in learning, improve students’ data analysis ability, and provide a better way to cultivate applied and innovative talents.
© The Authors, published by EDP Sciences, 2021
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