| Issue |
E3S Web Conf.
Volume 254, 2021
International Scientific and Practical Conference “Fundamental and Applied Research in Biology and Agriculture: Current Issues, Achievements and Innovations” (FARBA 2021)
|
|
|---|---|---|
| Article Number | 03005 | |
| Number of page(s) | 7 | |
| Section | Methods and Techniques of Genetic Engineering in the Creation of New Forms of Plants | |
| DOI | https://doi.org/10.1051/e3sconf/202125403005 | |
| Published online | 05 May 2021 | |
Comparative estimation of metrological characteristics of different PCR types for quantitative material assessment in complex matrices on the example of soybeans’ GM line
The Russian State Center for Animal Feed and Drug Standardization and Quality (FGBU «VGNKI»), Moscow, Russia
* Corresponding author: o.prasolova@vgnki.ru
A method of quantitative determination of genetically modified line (GM line) of soybean Mon 89788 based on digital PCR has been developed. Comparative analysis of metrological characteristics of this technique with identical based on PCR-RT was carried out. Both the detection limit and the quantitative detection limit of the methodology were defined. Correlation coefficient (R2=0.9987) was close to the maximum value, indicating a good correlation of the analytical signal linear dependence of the GMO content in the panel samples. The digital PCR methodology allows to determine the GMO content in the range from 0.5% to 100%. Compared to real time PCR results, digital PCR results show GMO content closer to the reference value; however, this technique does not allow to determine GMO content below 0.5%.
© The Authors, published by EDP Sciences, 2021
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