| Issue |
E3S Web Conf.
Volume 271, 2021
2021 2nd International Academic Conference on Energy Conservation, Environmental Protection and Energy Science (ICEPE 2021)
|
|
|---|---|---|
| Article Number | 02022 | |
| Number of page(s) | 5 | |
| Section | Environmental Protection and Green Application of Energy Saving and Emission Reduction | |
| DOI | https://doi.org/10.1051/e3sconf/202127102022 | |
| Published online | 15 June 2021 | |
Application of digital PCR (dPCR) in the detection of Covid-19 in food
1 Guangzhou College of Technology and Business, Guangzhou, China, 510850
2 University Putra Malaysia, 43400 Serdang, Selangor, Malaysia
Corresponding author: annylh2008@126.com
Covid-19 detection in food is an effective solution to ensure the accurate detection rate of Covid-19. The difficulties and detection methods of food virus safety detection and the feasibility of digital PCR detection technology are analyzed. The main parameters and characteristics of dPCR technology and other PCR technologies are compared. The application of dPCR technology in the detection of food viruses and pathogenic bacteria, the application of dPCR technology in the preparation and purity verification of Covid-19 RNA reference material, and the steps and methods of dPCR technology in food testing Covid-19 were expounded. Compared with traditional detection methods, digital PCR technology has great advantages in virus detection limit and stability. dPCR will develop towards high flux and automation, and achieve the absolute quantification of multiple target sequences at low cost. It will help to play a crucial role in the detection of covid-19 in food.
© The Authors, published by EDP Sciences, 2021
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