| Issue |
E3S Web Conf.
Volume 271, 2021
2021 2nd International Academic Conference on Energy Conservation, Environmental Protection and Energy Science (ICEPE 2021)
|
|
|---|---|---|
| Article Number | 03014 | |
| Number of page(s) | 4 | |
| Section | Research on Energy Chemistry and Chemical Simulation Performance | |
| DOI | https://doi.org/10.1051/e3sconf/202127103014 | |
| Published online | 15 June 2021 | |
Oridonin, an AKT1 Inhibitor, Suppresses the Growth of Breast Cancers with Hyperactivation of PI3K/AKT Signaling
School of Clinical Medicine, Weifang Medical University, Weifang 261053, Shandong, China
* Corresponding author: lizizhang926@163.com
Purpose: The study investigates the therapeutic effect of Oridonin on PTEN loss and HER2 amplification PI3K/AKT signaling hyper-activated (p-AKTHigh) breast cancer. Methods: The experiment uses MDAMB231, MCF-10A, SKBR3 (HER2 amplification), MDAMB468 (PTEN loss) cells, and Xenograft Murine Models. Cell viability is assessed by MTT, Clonogenic growth, CCK-8 assays. The inhibition of phosphorylation is tested by Western blot. Xenograft studies are used to test tumor growth blockage.The most possible result: Oridonin inhibits phosphorylation of AKT1 substrates and downstream proteins instead of AKT1. Also, it blocks the growth of PTEN loss and HER2 amplification p-AKTHigh breast cancer. Conclusion: This study provides important information for the clinical trial of Oridonin. Future experiments should focus on its toxicity and efficacy.
© The Authors, published by EDP Sciences, 2021
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