E3S Web Conf.
Volume 290, 20212021 3rd International Conference on Geoscience and Environmental Chemistry (ICGEC 2021)
|Number of page(s)||6|
|Section||Environmental Chemistry and Chemical Technology Application|
|Published online||14 July 2021|
Three basic types of fluorescence microscopy and recent improvement
1 Research School of Chemistry, Australia National University, Canberra, ACT, 2601, Australia
2 Guangzhou Foreign Language School, Guang Zhou, Guang Dong, 511455, China
† These authors contributed equally.
Corresponding author’s e-mail:email@example.com
Fluorescence microscopy is one of the most used imaging modalities in molecular biology and living specimens. To increase image contrast and spatial resolution, different type of fluorescence microscopy has been developed. This review introduces three main types of fluorescence microscopy: wild-field microscopy, confocal microscopy, and total internal reflection fluorescence microscopy. The basic principles are similar but with different modifications, which also indicates their attributes and limitation. The recent improvement on these microscopies is also discussed. Some most recent techniques show advance in overcoming common fluorescence microscopy's weakness, and future perspectives are also discussed.
© The Authors, published by EDP Sciences, 2021
This is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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