E3S Web Conf.
Volume 319, 2021International Congress on Health Vigilance (VIGISAN 2021)
|Number of page(s)||6|
|Published online||09 November 2021|
Bio contamination of surfaces and medical devices in controlled environment areas at a hospital in Morocco
1 Laboratory of Microbial Biotechnology & Bioactive Molecules, Faculty of Science and Technology, Sidi Mohammed Ben University Abdeallah Fez. BP 2202, Fes, Morocco.
2 Higher Institut of Nursing and Health Professions of Fez-Meknes. Regional Directorate of Health Fes-Meknes, El Ghassani Hospital, Dhar El Mehraz, 30000 Fes, Morocco.
3 Medical Analysis Laboratory of the Meknes Hospital Center, Regional Health Department Fes-Meknes.
4 Hassan First University, Higher Institute of Health Sciences, Health sciences and technologies laboratory, Casablanca Road km 3, 5 BP 555 Settat – Morocco.
* Corresponding author:firstname.lastname@example.org
The hospital environment, especially medical devices and surfaces, represents a secondary reservoir for pathogens. This work aims to evaluate the microbiological quality of surfaces and medical equipment of controlled environment services (burn unit, operating room, and sterilization service) at a hospital in Meknes (center of Morocco). This study was carried out for three months (September-December of 2017). A total of 63 samples were taken by swabbing technique from different surfaces and medical equipment after bio-cleaning. Identification was performed according to conventional bacteriological methods and by microscopic observation for fungi. The study showed that 68% of the surface was contaminated. The operating room recorded a rate of 93% of contamination (p-value <0.01), 83% for sterilization service, and 47% for burn unit. A percentage of 67% of the isolates were identified as Gram-positive bacteria against 32% Gram-negative bacteria (p-value <0.05). Bacterial identification showed Coagulase-negative Staphylococci (45%), Enterobacter cloacae (14%), Micrococcus sp (10%), Klebsiella pneumoniae, peptostreptococcus sp and Pseudomonas fluorescens (7% for each one), Escherichia coli, and Methicillin-resistant Staphylococcus aureus (5% for each one). These results require corrective action represented by rigorous cleaning and disinfection procedures.
© The Authors, published by EDP Sciences, 2021
This is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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