| Issue |
E3S Web Conf.
Volume 335, 2022
The 2nd International Conference on Environmentally Sustainable Animal Industry (The 2nd ICESAI 2021)
|
|
|---|---|---|
| Article Number | 00043 | |
| Number of page(s) | 5 | |
| DOI | https://doi.org/10.1051/e3sconf/202233500043 | |
| Published online | 14 January 2022 | |
Associations between Lactoferrin (LTF) gene polymorphism exon 4 and milk compositions in Senduro goat
Faculty of Animal Science, Universitas Brawijaya, Malang 65145, East Java, Indonesia
* Corresponding author: triekos@ub.ac.id
This research aimed to analyze the polymorphism of Lactoferrin (LTF) gene exon 4 associated with milk compositions in Senduro goats. A total of 42 DNA samples and milk compositions from Senduro goats were used in this study. The DNA sequence was amplified using Polymerase Chain Reaction (PCR) with a pair of primers. Genotyping was carried out using DNA sequencing and analyzed using FinchTV 1.4.0 and MEGA 6.0. In this research, the results showed that there were three genotypes (CC, CT, and TT) and two alleles (C and T). The frequencies of CC, CT, and TT genotypes were 0.381; 0.452; and 0.167, respectively. Furthermore, the frequencies of C and T alleles were 0.607 and 0.393, respectively. The genotype polymorphism did not affect on milk compositions. In conclusion, there was no association between polymorphism of LTF gene exon 4 and milk compositions in Senduro goats.
© The Authors, published by EDP Sciences, 2022
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