E3S Web Conf.
Volume 335, 2022The 2nd International Conference on Environmentally Sustainable Animal Industry (The 2nd ICESAI 2021)
|Number of page(s)||5|
|Published online||14 January 2022|
A review chromosome analysis comparison of swamp buffalo (Bubalus bubalis) with different phenotypic characters in East Java, Indonesia
1 Faculty of Animal Science, Brawijaya University, 65145 Malang, Indonesia
2 Faculty of Mathematics dan Science, Biology Department, Brawijaya University, 65145 Malang Indonesia
3 Central laboratoium (LSIH), Brawijaya University, 65145 Malang, Indonesia
4 Biosains Institute, Brawijaya University, 65145 Malang, Indonesia
5 Faculty of Animal Husbandry, Islamic University of Malang, 65144 Malang, Indonesia
6 Faculty of Veterinary Medicine, Brawijaya University, 65145 Malang, Indonesia
* Corresponding author: email@example.com
Chromosomal data on swamp buffalo especially in East Java province, is still lacking. For breeders, chromosomal analysis needs to be done to detect the possibility of hereditary genetic defects. In Malang Area, farmers lack of care and breeding programs for swamp buffalo also caused a decline in the genetic quality and performance. The purpose of this study was to compare the results of the chromosome analysis of swamp buffalo in Malang and another area in East Java province, which were selected based on differences in phenotypic characters. Chromosome analysis is very important for breeders because the results of this study can be used as a consideration for strategies to improve the genetic quality of swamp buffalo. The method used is standard karyotyping using whole blood with G banding staining. Chromosomal preparation using Karyo MAX medium, Colcemic Solution, Giemsa Stain, and KCl solution. Cell culture was performed according to the standard karyotyping method in mammals. The minimum number of 5 spreading chromosomes is the best was chosen, microphotographed, and then chromosome analysis is performed using Cytovision Image Analysis software. The results of this study did not find swamp buffalo with an abnormal number of chromosomes in East Java Province, Indonesia.
© The Authors, published by EDP Sciences, 2022
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