| Issue |
E3S Web Conf.
Volume 363, 2022
XV International Scientific Conference on Precision Agriculture and Agricultural Machinery Industry “State and Prospects for the Development of Agribusiness - INTERAGROMASH 2022”
|
|
|---|---|---|
| Article Number | 03068 | |
| Number of page(s) | 10 | |
| Section | Precision Agriculture. Yield Monitoring and Estimation | |
| DOI | https://doi.org/10.1051/e3sconf/202236303068 | |
| Published online | 14 December 2022 | |
The possibility of using Ig Y-antibodies in immunotherapy
A. S. Pushkin Leningrad State University (Luga branch), Volodarskogo str., 52a, Luga of the Leningrad region, 188230, Russia
* Corresponding author: sboich.viktor@yandex.ru
The importance of yolk antibodies (IgY) for their use in the diagnosis and treatment of human diseases is shown. The structural features of IgY compared to human immunoglobulins were studied. The disadvantages of obtaining hyperimmune sera from mammalian animals were revealed. A feature of IgY is its resistance to trypsin and chymotrypsin, which can contribute to more effective treatment of diseases. Data on optimization of the technology for obtaining yolk antibodies (IgY) from various bird species are presented. It is shown that 25-50 mg of highly purified immunoglobulin can be obtained from one bird egg. Obtaining immunoglobulins from eggs is more humane than using mammals for this purpose. Compared to ion exchange chromatography, gel chromatography, and ultrafiltration, the methods of precipitation with various chemicals (ammonium sulfate) were found to be simpler in terms of methodology. The results of electrophoresis revealed two types of proteins with a molecular weight of about 65 kDa and 30 kDa, which corresponds to the mass of the «heavy» and «light» IgY chains. Quail species Coturnix coturnix japonica as a producer of biological raw materials for the production of diagnostic immunoglobulins have great advantages over chickens (Gallus domesticus).
© The Authors, published by EDP Sciences, 2022
This is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Current usage metrics show cumulative count of Article Views (full-text article views including HTML views, PDF and ePub downloads, according to the available data) and Abstracts Views on Vision4Press platform.
Data correspond to usage on the plateform after 2015. The current usage metrics is available 48-96 hours after online publication and is updated daily on week days.
Initial download of the metrics may take a while.