| Issue |
E3S Web of Conf.
Volume 374, 2023
The 3rd International Conference on Natural Resources and Life Sciences (NRLS) 2020
|
|
|---|---|---|
| Article Number | 00025 | |
| Number of page(s) | 6 | |
| DOI | https://doi.org/10.1051/e3sconf/202337400025 | |
| Published online | 21 March 2023 | |
Influence of Hibiscus tilliaceus Extract and Suspension on Echerichia coli and Staphylococcus aureus Growth
1 Pharmacy Academy of Putra Indonesia Malang, Jl. Barito No.5, Malang 65123, East Java, Indonesia
2 Faculty of Science and Data Analytics, Sepuluh Nopember Institute of Technology (ITS), Kampus ITS Sukolilo, Surabaya 60111, East Java, Indonesia
3 Nazar College of Pharmacy, Dakson Institue of Health Sciences, Main Murree Road, Barakahu, 45400 Islamabad, Pakistan
* Corresponding author: oktavina.chemistry@gmail.com
This research aims to know the influence of suspension compared to its crude extract on antibacterial activity, especially for Escherichia coli and Staphylococcus aureus. An independent t-test was used as statistical analysis. The yield of the thick extract obtained was 11.153 % ± 1.032 % and it gave an inhibition zone of 7.878 mm ± 0.301 mm for Escherichia coli and 7.484 mm ± 0.636 mm for Staphylococcus aureus. While the suspensions gave an inhibitory zone of 6.293 mm ± 0.754 mm and 5.140 mm ± 0.121 mm for Escherichia coli and Staphylococcus aureus in sequence. The extract’s particle size followed by suspension was 951.167 mm ± 113.662 nm and 474.000 mm ± 38.500 nm. The independent t-test results showed that the extract and suspension significantly influenced the growth of Staphylococcus aureus and Escherichia coli with sig values of 0.012 and 0.028. In conclusion, the extract had a greater effect than the suspension on the growth of Staphylococcus aureus and Escherichia coli.
Key words: Antibacterial effect / effectiveness extract / optimal extract activity / particle size / suspension
© The Authors, published by EDP Sciences, 2023
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