| Issue |
E3S Web Conf.
Volume 151, 2020
The 1st International Conference on Veterinary, Animal, and Environmental Sciences (ICVAES 2019)
|
|
|---|---|---|
| Article Number | 01029 | |
| Number of page(s) | 5 | |
| DOI | https://doi.org/10.1051/e3sconf/202015101029 | |
| Published online | 14 February 2020 | |
Potential of Malacca leaf (Phyllanthus emblica) against Salmonella sp
1
Study Program Doctor Mathematical Applied Science Universitas Syiah Kuala, Banda Aceh 23111, Indonesia
2
Clinical Laboratory Veterinary Medicine Universitas Syiah Kuala, Banda Aceh 23111, Indonesia
3
Microbiology Laboratory Universitas Syiah Kuala, Banda Aceh 23111, Indonesia
4
Parasitology Laboratory Universitas Syiah Kuala, Banda Aceh 23111, Indonesia
5
Pharmacology Laboratory Universitas Syiah Kuala, Banda Aceh 23111, Indonesia
6
Veterinary Study Program, Faculty of Veterinary Medicine, Universitas Syiah Kuala, Banda Aceh 23111, Indonesia
* Corresponding author: nuzulasmilia@unsyiah.co.id
Malacca is one of traditional medicine that possesses a potent antimicrobial activity. This study aims to determine the inhibitory activity of Malacca leaf extract on the growth of Salmonella sp in vitro. The bacteria was obtained from Microbiology Laboratory of the Faculty of Veterinary Medicine, Universitas Syiah Kuala. The study was conducted using n-hexane extract, ethyl acetate extract and ethanol of malacca leaves with dilution concentrations of 5%, 25%, and 50%.The inhibitory property of malacca leaf was tested using Kirby-Bauer method. Data were analyzed descriptively. The results of this study indicate that n-hexane extract, ethyl acetate extract and ethanol extract of malacca leaves can inhibit the growth of Salmonella sp. The n-hexane extract of malacca leaves showed a greater inhibition than the ethyl acetate and ethanol extract of malacca leaves. n-hexane extract with a concentration of 5%, 25%, and 50% showed average diameter inhibition of 1.35 mm (weak), 4.97 mm (moderate), and 12.87 mm (strong), respectively ethyl acetate extract with a concentration of 5%, 25%, and 50% showed average diameter inhibition of 2.00 mm (weak), 5.72 mm (moderate), and 7.58 mm (moderate), whereas in ethanol extract were 0.47 mm (weak), 2.58 mm (weak), and 4.35 mm (weak), repectively. The clear zone areas in negative and positive control were 0.00 mm 20.00 mm, respectively. Malacca leaf extract possess inhibitory property against the growth of the Salmonella sp.
Key words: Malacca leaves / Salmonella sp / Kirby-Bauer Method
© The Authors, published by EDP Sciences, 2020
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