Use of 2-aminoethanesulfonic acid (taurine) for cryopreservation and storage of Siberian sturgeon sperm

¹ Branch for the freshwater fisheries of “VNIRO” (“VNIIPRKH”), 40а, Rybnoe, Moskovskaya oblast’, 141821, Russia,
² Federal Research Centre the Southern Scientific Centre of the Russian Academy of Sciences, 41, Chekhov str., Rostov-on-Don, 344006, Russia
³ Don State Technical University, 1 Gagarin square, Rostov-on-Don, 344003, Russia

^* Corresponding author: silur5@mail.ru

Abstract

The research aimed to determine the effect of different taurine concentrations on the duration of sperm fertility preservation and the results of further use during cryopreservation. The taurine was injected into the sperm of the Siberian sturgeon by various methods under conditions of low positive temperatures. Immediately after preparation, the taurine was injected into native sperm at concentrations of 0.01, 0.05, and 0.1 mmol/ml. Two methods of introducing taurine into native sperm were tested: 1 (dry method) - adding taurine powder directly to native sperm and 2 (wet method) - adding taurine dissolved in saline to native sperm. The studies have shown that injections of taurine in an amount of 0.05 - 0.1 mmol/ml both dry and wet can be recommended as the method of long-term preservation of the native sperm of Siberian sturgeon viability. While storing Siberian sturgeon sperm for further cryopreservation the optimal taurine concentration is 0.01 mmol/ml.