Preliminary results of in-vitro laboratory propagation of barberry (Berberis L.)

¹ Tashkent State Agrarian University, University str., 2, 100140, Tashkent, Uzbekistan
² Scientific-Research Institute of Forestry, Darkhon, 702017, Tashkent, Uzbekistan
³ Tashkent Branch of The Samarkand State Universitety Veterinary Medicine of Livestock and Biotexnologies, Chilanzar avenue 25, Tashkent, Uzbekistan

^* Corresponding author: farkhodch@gmail.com

Abstract

The genus Berberis vulgaris (Berberis L.) is currently found in tropical, subtropical, temperate, and cold climate zones. There is little doubt that Southeast Asia is where the majority of Berberis vulgaris' interspecies biodiversity is found. Additionally, species are widely distributed, mostly in hilly areas, and, in terms of moisture requirements, they are mesophytic and xeromesophytic plants. In this article, experiments on in-vitro propagation of Berberis vulgaris (Berberis L.) seedlings were carried out in laboratory conditions. During the experiments, the time of sterilization of the explants obtained from the Berberis vulgaris plant was determined. Different concentrations of hormones and auxins were used in several nutrient mediums for growing in artificial nutrient medium. The results depicted that the highest rate was observed in the variant, where 2ml.BAP+0.5NAA+30g sucrose+7g accounted for 87% of plant rooting rate. It was observed that the rooting rate was slightly higher in the control variant of WPM-Woody plant medium-nutrient medium than the control variant of the MS nutrient medium, which was 17%.