Obtaining sterile Calligonum aphyllum material for in vitro culture preservation

Federal Scientific Center for Agroecology, Integrated Land Reclamation and Protective Afforestation of the Russian Academy of Sciences, Laboratory of Biotechnologies, Universitetsky Avenue 97, Volgograd, 400062, Russia

^* Corresponding author: mogilevskaya-i@vfanc.ru

Abstract

The comparative evaluation’s results on the efficiency of surface treatment of Calligonum aphyllum (Pall.) Guerke explants, a perspective plant species in the restoration of degraded lands, are presented. Sterilizers «Lysoformin 3000», silver nitrate, hydrogen peroxide, and «Belizna-econom» are used at the introduction stage of the plants in vitro. As a result, 2 of 11 effective sterilization modes were found when C. aphyllum was introduced into the culture in vitro: No. 10 (hydrogen peroxide solution 10% v/v, 5 min) and No. 11 (hydrogen peroxide solution 6% v/v, 15 min). These modes had the highest rates of sterile and regenerative explants. The microflora was also isolated from the studied explant samples. Determined the preparation «Lysoformin 3000» effectively acts on all isolated microorganisms. «Belizna-econom» and hydrogen peroxide (concentrations of 10% v/v) do not act on 80% of isolated bacterial strains. Shown the silver nitrate solution was not sensitive in 100% of cases. The obtained information can be used to draw up a complete protocol for C. aphyllum explant sterilization at the introduction stage in vitro.