An Overview- Advances in Chromatographic Techniques in Phytochemistry

Department of Chemistry, University Institute of Sciences, Chandigarh University, Mohali 140413, Punjab, India

^* corresponding author: anuradha.appsci@cumail.in

Abstract

The basic idea of the Chromatographic process is that the distinct compounds have different properties like solubility, adsorption, ion exchange, and affinity that can be regulated through various separation mechanisms causing the analytes to travel through the stationary phase at different speeds, resulting in their separation from a complex mixture. The Chromatographic techniques have been explored as multidimensional, which has been growing and evolving due to continuous advancements in technology allowing it to meet the upcoming challenges and analytical demands in various scientific scenarios including academics and industries. Phytochemistry is one of the oldest core areas of research in science where one of the major challenges in drug discovery from natural sources is the identification and isolation of closely related active molecules within these complex to the observed biological activities therefore there is utmost need to better understand their intrinsic complexity and exploit their vast commercial potential. Nowadays the hyphenated techniques with one component as the chromatographic technique have been widely explored as inevitable analytical tools for early detection and identification of bioactive compounds and driving forces in the evolution and discoveries in phytochemistry from crude plant extracts that boosted mainly due to rapid technological advancements in instrumentation. It’s also significant as an intellectual method for phytotherapeutic quality control and regulation. In the present review, we have discussed the advances and insight of chromatographic techniques which can be explored for comprehensive chemical profiles of herbal medicine preparations or extracts in phytochemistry. The review provides a systematic update of the recent advancement and published approaches to methodology in phytochemistry.