Antibiofilm Potential of Pisang Batu Extract (Musa balbisiana Colla) for Staphylococcus aureus Bacteria

Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Gorontalo, Jl. Prof. Dr. Bj Habibie, 96554, Indonesia

^* Corresponding author: syam_bio@ung.ac.id

Abstract

Phytochemical compounds present in Pisang Batu (Musa balbisiana Colla) can inhibit the growth of bacteria on the surface of a film. This study aims to determine the effect of the anti-biofilm capacity of Pisang Batu extract ( Musa balbisiana Colla) on Staphylococcus aureus bacteria and to determine significant differences in the antibiofilm capacity of Pisang Batu extract (Musa balbisiana Colla) against Staphylococcus aureus bacteria. This study used an experimental method consisting of 6 treatments with 4 replications. The treatment group was a concentration of 25%, 50%, 75%, 100%, positive control (Ciprofloxacin ), and negative control (CMC 1%). Measurement of biofilm inhibition was obtained from the reading of the Optical Density Value on the tool spectrophotometer which then calculated the percentage inhibition biofilm. Data were analyzed using the One Way Anova Test and then Duncan’s Post Hoc test was carried out. The results showed that Pisang Batu extract had an effect on the antibiofilm effect of Pisang Batu extract (Musa balbisiana Colla) on Staphylococcus aureus bacteria where at a concentration of 100% an inhibition percentage value of 39.8% was obtained, a concentration of 75% obtained an inhibition percentage value of 58.03%, 50% concentration obtained inhibition percentage value of 28.11%, 25% concentration obtained inhibition percentage value of 18.06%, control positive (Ciprofloxacin ) obtained an inhibition percentage value of 95.19% and negative control (CMC 1%) obtained an inhibition percentage value of 0%. Then after further testing, a significant difference was obtained for all treatment groups with the best concentration obtained at a concentration of 75%.