Bacterial flora of infected vegetable crops

¹ Scientific Research Institute of Microbiology of the Academy of Sciences of Uzbekistan, Tashkent, 100030, Uzbekistan
² Tashkent State Agrarian University, Tashkent, 100140, Uzbekistan
³ Karakalpakstan Agricultural Institute, Nukus, 230100, Uzbekistan

^* Corresponding author: nodira.azimova1001@mail.ru

Abstract

The focal point of the article revolves around the examination of bacteria isolated from infected vegetable crops. The study encompasses a comprehensive analysis of various attributes including colony structure, color, and size of these isolated bacteria in their pure form. To enhance our understanding, fixed preparations were meticulously crafted from these isolates, followed by Gram staining. The isolates were scrutinized under immersion oil using an x 100 lens and specialized microscopy equipment, namely the xsp-136 B and N-300m microscope (UCMOSO9000KPB), magnifying them up to 1000 times. To further delve into the taxonomic classification of these isolated strains, the MALDI-TOF method was employed. The strains were precisely identified through this method, carried out at the Department of Sanitary and Epidemiological Control, utilizing state-of-the-art Bruker equipment. Central to the research was the exploration of the pathogenic properties of these bacteria in relation to plants. To this end, a pivotal experiment was conducted wherein seeds of tomatoes and eggplants were artificially planted using these isolated bacteria. The culmination of these investigations has resulted in the creation of four distinct tables, meticulously supplemented with figures to visually encapsulate the findings. In essence, the article presents a comprehensive exploration of the isolated bacteria’s attributes, classification, and their potential pathogenic impact on plants.