| Issue |
E3S Web Conf.
Volume 460, 2023
International Scientific Conference on Biotechnology and Food Technology (BFT-2023)
|
|
|---|---|---|
| Article Number | 01001 | |
| Number of page(s) | 6 | |
| Section | Food Microbiology and Safety | |
| DOI | https://doi.org/10.1051/e3sconf/202346001001 | |
| Published online | 11 December 2023 | |
Inoculum for the cultivation of Methylosinus sporium
Saint-Petersburg State Institute of Technology, 26 Moskovsky Prospect, 190013 St. Petersburg, Russia
* Corresponding author: uliana.kononova@mail.ru
This research assessed the effect of the amount of inoculum on the specific growth rate of Methylosinus sporium culture. Methanotrophs were inoculated in different quantities onto media of the same composition and volume. Seven different initial inoculum concentrations were obtained in duplicate. Samples were cultured for 31 hours, during which sampling and optical density measurements were carried out. In accordance with the fact that the optical density is directly proportional to the concentration of microorganisms in the sample, the specific growth rate was calculated. The research revealed an interval from 6.5 to 24 hours, during which the culture demonstrates a stable growth rate of 0.06 h-1, at an inoculation dose at which the optical density of the nutrient medium with the added inoculum is 0.030-0.121.
© The Authors, published by EDP Sciences, 2023
This is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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