| Issue |
E3S Web Conf.
Volume 460, 2023
International Scientific Conference on Biotechnology and Food Technology (BFT-2023)
|
|
|---|---|---|
| Article Number | 01002 | |
| Number of page(s) | 6 | |
| Section | Food Microbiology and Safety | |
| DOI | https://doi.org/10.1051/e3sconf/202346001002 | |
| Published online | 11 December 2023 | |
Inoculum for the cultivation of Methylosinus trichosporium
Saint-Petersburg State Institute of Technology, 26 Moskovsky Prospect, 190013, St. Petersburg, Russia
* Corresponding author: latypovaulyanatwp@gmail.com
In this study, the effect of the amount of introduced Methylosinus trichosporium culture inoculum on the specific growth rate was evaluated in order to improve the cultivation process. The variation of the studied factor allowed us to establish the dependence and determine the optimal dose of the inoculum for the culture in question. The prepared samples containing a nutrient medium with different inoculate content were cultured for 48 hours, during which the trend of changes in the optical density of the samples was studied. Based on the fact that the optical density is directly proportional to the concentration of microorganisms in the samples, their specific growth rate was calculated. The study showed that the culture of Methylosinus trichosporium demonstrates a stable growth rate in the range from 3 to 24 hours of cultivation, at such an inoculation dose at which the optical density of the nutrient medium with the introduced inoculum is 0.07-0.55. The data obtained provide tools for regulating the process of cultivation of Methylosinus trichosporium methanotrophs.
© The Authors, published by EDP Sciences, 2023
This is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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