Issue |
E3S Web Conf.
Volume 588, 2024
Euro-Asian Conference on Sustainable Nanotechnology, Environment, & Energy (SNE2-2024)
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Article Number | 02008 | |
Number of page(s) | 11 | |
Section | Nanomaterials in Environment and Energy | |
DOI | https://doi.org/10.1051/e3sconf/202458802008 | |
Published online | 08 November 2024 |
Silver Nanoparticles in Biomedical Applications: Insights from In Vitro and In Vivo Studies
1 Centre of Research Impact and Outcome, Chitkara University, Rajpura - 140417, Punjab, India,
2 Chitkara Centre for Research and Development, Chitkara University, Himachal Pradesh - 174103 India
3 Department of Computer Engineering & Application. GLA University, Mathura (U.P) - 281406
4 Lovely Professional University, Phagwara, Punjab, India,
5 Uttaranchal University, Dehradun - 248007, India
6 Department of Mechanical, GRIET, Bachupally, Hyderabad, Telangana, India.
7 University School of Social Sciences, Research & Incubation Centre, Research & Incubation Centre, Rayat Bahra University, Chandigarh-Ropar NH 205, Greater Mohali, Punjab, 140103, India
8 University Research Department, Bahra University, Waknaghat, Distt. Solan, HP-173234, India
9 Department of Structurals Techniques engineering, College of technical engineering, The Islamic University of Al Diwaniyah, Al Diwaniyah, Iraq
* Corresponding author: shriya.mahajan.orp@chitkara.edu.in
This work provides a thorough examination of the biological impacts of silver nanoparticles (AgNPs) via in vitro and in vivo investigations. In vitro tests demonstrated a concentration- dependent reduction in cell viability, decreasing from 90% at 5 µg/mL to 50% at 25 µg/mL, with a substantial rise in reactive oxygen species (ROS) from 20% to 80%. The data indicate that AgNPs may possess cytotoxic effects. Moreover, apoptosis rates escalated from 5% to 18% with increasing doses of AgNP. In vivo investigations revealed significant alterations in hematological parameters, including a decrease in hemoglobin concentrations (from 15 g/dL to 11 g/dL), an elevation in white blood cell counts (from 8 x10³/µL to 12 x10³/µL), and a drop in platelet counts (from 250 x10³/µL to 200 x10³/µL) corresponding to escalating AgNP dosages. Histological studies demonstrated dose-dependent changes in organ tissues, especially the liver, with scores escalating from two to six. Tumor size reductions of 20%, 40%, and 60% were seen with low, medium, and high dosages of AgNPs, respectively. These results underscore the promise of AgNPs for biological applications, especially in tumor therapy. Nonetheless, comprehensive safety evaluations and accurate dosage optimization are required prior to their clinical use. This work highlights the potential biological uses of AgNPs while stressing the need of meticulous evaluation to guarantee their safe and effective incorporation into medical procedures.
Key words: Biomedical applications / silver nanoparticles / in vitro experiments / in vivo assessments / cytotoxic effects
© The Authors, published by EDP Sciences, 2024
This is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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