Issue |
E3S Web Conf.
Volume 503, 2024
The 9th International Symposium on Applied Chemistry in conjuction with the 5th International Conference on Chemical and Material Engineering (ISAC-ICCME 2023)
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Article Number | 07010 | |
Number of page(s) | 9 | |
Section | Organic and Natural Product Chemistry | |
DOI | https://doi.org/10.1051/e3sconf/202450307010 | |
Published online | 20 March 2024 |
Optimization of Loop-Mediated Isothermal Amplification Reaction for Detecting Streptococcus pneumoniae Serotypes in Clinical Nasopharynx Swab Samples
1 Research Center for Pharmaceutical Ingredients and Traditional Medicine, National Research and Innovation Agency (BRIN). KST B.J. Habibie Gedung 452, Serpong, Tangerang Selatan, Banten 15314, Indonesia
2 Eijkman Reserch Center for Molecular Biology, National Research and Innovation Agency (BRIN) Komplek Cibinong Science Center – BRIN. Jln. Raya Bogor Km 46, Cibinong 16911, Jawa Barat, Indonesia
3 Research Center for Applied Microbiology, National Research and Innovation Agency (BRIN). KST Samaun Samadikun, Jl. Cisitu-Sangkuriang, Bandung 40135, Jawa Barat, Indonesia
* Corresponding author: tjandrawati@brin.go.id
Current molecular PCR-based methods for identifying Streptococcus pneumoniae, the primary cause of pneumonia, meningitis, and other invasive diseases, are accurate but require expensive infrastructure and have a long run time, which restricts their use, particularly in developing countries. LAMP, or loop-mediated isothermal amplification, is a low-cost alternative to polymerase chain reaction (PCR) that can be used quickly because the reaction only takes place at a constant temperature. We aimed to develop a LAMP assay for rapid detection of Streptococcus pneumoniae serotypes in nasopharynx swab samples. The LAMP primers were designed using a conserved region of the lytA gene. An incubation time range of 30 to 60 minutes was studied to optimize the LAMP reaction. The real-time fluorescence intensity was monitored during the amplification reaction. Clinical nasopharynx swab samples identified as Streptococcus pneumoniae and their serotypes were tested to evaluate the performance of LAMP. To investigate the specificity of the LAMP, Streptococcus species samples and non-Streptococcus species samples were analyzed. In conclusion, the optimized LAMP assay is capable of detecting Streptococcus pneumoniae and its serotypes in nasopharynx swab samples.
© The Authors, published by EDP Sciences, 2024
This is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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