| Issue |
E3S Web Conf.
Volume 503, 2024
The 9th International Symposium on Applied Chemistry in conjuction with the 5th International Conference on Chemical and Material Engineering (ISAC-ICCME 2023)
|
|
|---|---|---|
| Article Number | 09003 | |
| Number of page(s) | 13 | |
| Section | Surface Chemistry and Nanoparticles | |
| DOI | https://doi.org/10.1051/e3sconf/202450309003 | |
| Published online | 20 March 2024 | |
Comparative study of well diffusion and disc diffusion method to investigate the antibacterial properties of silver nanoparticles synthesized from Curcuma longa extracts
1 Research Centre for Chemistry, National Research and Innovation Agency (BRIN), Kawasan Puspiptek, Building 452, Serpong, Tangerang Selatan, Banten 15314 Indonesia
2 Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Negeri Semarang, Semarang, Central Java 50229 Indonesia
3 Department of Chemical Engineering, Faculty of Engineering, Diponegoro University, Semarang, 50275, Indonesia
4 Research Centre for Pharmaceutical Ingredients and Traditional Medicine, National Research and Innovation Agency (BRIN), Kawasan Puspiptek Serpong, Tangerang Selatan, Banten 15314 Indonesia
* Corresponding author: agus147@brin.go.id
Green synthesis method for the preparation of silver nanoparticles (AgNPs) using Curcuma longa extracts as reducing and stabilizing agents was studied. The optimization of the synthesis was determined by varying the weight of the extracts, the AgNO3 concentration, and the reaction time. The results revealed that 3 g of extracts, 0.005 M AgNO3, and 30 min of incubation time were the ideal conditions to synthesize AgNPs-Cur. The characterization was assessed by UV-Visible spectroscopy, FTIR, and TEM. UV-Vis spectra showed a surface plasmon resonance (SPR) peak at 428 nm with the stability after 5 months of storage time. FTIR analysis revealed the formation of AgNPs using turmeric extracts and the synthesized AgNPs-Cur had the average size of 22.12 ± 0.60 nm (analyzed by TEM). The well diffusion and disc diffusion method were performed to investigate the antibacterial activity of AgNPs-Cur against Bacillus subtilis, Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa. The results concluded that AgNPs-Cur exhibited better bacterial growth inhibitory activities with well diffusion method than disc diffusion method.
© The Authors, published by EDP Sciences, 2024
This is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Current usage metrics show cumulative count of Article Views (full-text article views including HTML views, PDF and ePub downloads, according to the available data) and Abstracts Views on Vision4Press platform.
Data correspond to usage on the plateform after 2015. The current usage metrics is available 48-96 hours after online publication and is updated daily on week days.
Initial download of the metrics may take a while.