Acute in Vitro Exposure of Potassium Dichromate on Oxidative Stress, Cell Density, and Cell Viability of Osteochilus Vittatus

Faculty of Biology, Jenderal Soedirman University, Purwokerto, Central Java, Indonesia, 53122

^* Corresponding author: bugrat@gmail.com

Abstract

The harmful effect of K₂Cr₂O₇ has been reported in animals and humans but the mechanism in exerting such an effect is unclear. An experimental study applying a completely randomized design was conducted to evaluate the impact of in vitro exposure to K₂Cr₂O₇ on MDA level, cell density, and cell viability in Osteochilus. vittatus testis. The testis fragments were cultured in DMEM supplemented with 10% FBS and 5% pen-strep for 24 hours and 72 hours at 29°C; 5 concentrations of K₂Cr₂O₇: 0µg.mL^-1, 0.125 µg.mL-1, 0,250 µg.mL-1, and 0.50µg.mL-1 were tested. The result showed that the exposure of 0.500 µg.mL^-1 K₂Cr₂O₇ for 24 hours did not significantly affect the MDA level (p>0.05), but it significantly reduced cell density (p<0.05) and cell viability (p<0.01). Exposure to 0.500 µg.mL^-1 K₂Cr₂O₇ for 72 hours significantly increased the DMA level (p<0.05), cell density (p<0.05), and cell viability (0.01). These results indicated that K₂Cr₂O₇ induces oxidative stress, cell proliferation, and cell survival of Osteochilus vittatus testis.