E3S Web Conf.
Volume 67, 2018The 3rd International Tropical Renewable Energy Conference “Sustainable Development of Tropical Renewable Energy” (i-TREC 2018)
|Number of page(s)||8|
|Section||Multifunctional and Advanced Materials|
|Published online||26 November 2018|
Production of Plutella xylostella bioinsecticide for brassicaceae plant based on bromelain enzyme extracted from pineapple peel and isothiocyanate extracted from broccoli stem and radish peel
Department of Chemical Engineering, Faculty of Engineering, University of Indonesia, Depok, West Java, Indonesia
* Coresponding author: email@example.com
Brassicaceae plant is a commodity of edible plant that is very important for Indonesia, with cabbage worm (Plutella xylostella) as its main pest. Pest control with biopesticide is needed to preserve environment and increase crops production. Several biological substance is toxic to cabbage worm, including bromelain enzyme and isothiocyanates. Extraction of bromelain is experimented by varying mixing duration (15, 30, 45, 60 mins) and solvent type (distilled water and phosphate buffer). Extraction of isothiocyanate is experimented by varying the solvent ratio (1:1, 1:2, 1:3), using dichloromethane as solvent. For the broccoli stem sample, phosphate buffer is added to maintain pH. Enzyme activity test is used with product tyrosin concentration as standard. Isothiocyanate is determined with GC-MS analysis. The most effective extraction method for bromelain is with distilled water as solvent with 15 min mixing time. The most effective extraction method for isothiocyanate is with 1:1 solvent ratio at 37°C temperature without adding phosphate buffer. Efficacy of each sample to cabbage worm is also experimented by feeding it broccoli leaf spread with sample and observing the mortality rate. Bromelain and isothiocyanate proved to be toxic to cabbage worm and can be used as alternative bioinsecticide with the highest mortality of cabbage worm is by isothiocyanate from radish peel, reaching 100%.
© The Authors, published by EDP Sciences, 2018
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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