E3S Web Conf.
Volume 78, 20192018 International Seminar on Food Safety and Environmental Engineering (FSEE 2018)
|Number of page(s)||5|
|Section||Nutrition and Health, Medical Treatment and Biomedical Engineering|
|Published online||15 January 2019|
Studies on Ejection of Cell Culture Medium by Electrohydrodynamic Method
Faculty of Information Technology, Beijing University of Technology, Beijing 100124, China
2 Laser Engineering Research Institute, Beijing University of Technology, Beijing 100124, China
3 Beijing Institute of Pediatrics, Beijing Children's Hospital, Capital Medical University, Beijing 100035, China
* Corresponding author: email@example.com
Sample deposition based on micro-droplet ejection has broad application prospects in the field of biomedicine. Ejection of RPMI-1640 medium (without and with cells) is investigated experimentally using a home-build electrohydrodynamic (EHD) ejection system, consisting of a liquid supplier and a nozzle, a high voltage source, a droplet collector, and a high speed photography module. Electric voltage is applied between the nozzle and the droplet collector. The liquid surface is charged and the ejection takes place when electric force overcomes the surface tension. The ejection process is studied by using high speed photography and image processing. At low voltage, a uniform ejection state is established with ejection frequency typically less than 50Hertz. At sufficiently high voltage, another uniform ejection state is reached with ejection frequency as high as 1300Hz. Human peripheral blood mononuclear cells, after ejection, show survival rates higher than 79%, manifesting EHD ejection as a promising technique for cell printing.
© The Authors, published by EDP Sciences, 2019
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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