E3S Web Conf.
Volume 78, 20192018 International Seminar on Food Safety and Environmental Engineering (FSEE 2018)
|Number of page(s)||6|
|Section||Food Safety, Extraction and Food Engineering|
|Published online||15 January 2019|
Rapid Determination of Carbendazim Residues in Mushrooms by Immunosorbent Assay1
Tianjin Key Laboratory of Animal and Plant Resistance, College of Life Sciences, Tianjin Normal University, Tianjin 300387, China ;
2 National Engineering and Technology Research Center for Preservation of Agricultural Products, Tianjin Key Laboratory of Postharvest Physiology and Storage of Agricultural Products, Tianjin 300384, China ;
3 Tianjin Forestry and Fruit Research Institute, 300384.
* Corresponding author: Zhou Yong-bin: firstname.lastname@example.org
In this study, the testing time was reduced to 1 h through optimizing, and an LOD of 0.3±0.15 μg·L-1 and an IC50 of 2.7±0.3 μg·L-1 were achieved by the optimized CD-ELISA. The sample extraction of 1-fold acetonitrile followed by a 20-fold dilution in assay diluted buffer has proven to be sufficient to eliminate the influence of matrix. The CD-ELISA was validated further by comparing with the standard HPLC. The average recovery ratio of 89.7%~112.7% was obtained, and the coefficients of variation were less than 11.0%. In actual samples detection, except for Pleurotus eryngii, carbendazim was not detected, and other mushrooms were all detected, and only carbendazim in Volvariella volvacea exceeded the standard, and the over-standard rate was 16.7 %. In a word, the rapid, sensitive and efficient CD-ELISA for quantifing carbendazim in mushrooms was established in the study.
© The Authors, published by EDP Sciences, 2019
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