Rapid Determination of Carbendazim Residues in Mushrooms by Immunosorbent Assay¹

¹ Tianjin Key Laboratory of Animal and Plant Resistance, College of Life Sciences, Tianjin Normal University, Tianjin 300387, China ;
² National Engineering and Technology Research Center for Preservation of Agricultural Products, Tianjin Key Laboratory of Postharvest Physiology and Storage of Agricultural Products, Tianjin 300384, China ;
³ Tianjin Forestry and Fruit Research Institute, 300384.

^* Corresponding author: Zhou Yong-bin: 2438059329@qq.com

Abstract

In this study, the testing time was reduced to 1 h through optimizing, and an LOD of 0.3±0.15 μg·L^-1 and an IC50 of 2.7±0.3 μg·L^-1 were achieved by the optimized CD-ELISA. The sample extraction of 1-fold acetonitrile followed by a 20-fold dilution in assay diluted buffer has proven to be sufficient to eliminate the influence of matrix. The CD-ELISA was validated further by comparing with the standard HPLC. The average recovery ratio of 89.7%~112.7% was obtained, and the coefficients of variation were less than 11.0%. In actual samples detection, except for Pleurotus eryngii, carbendazim was not detected, and other mushrooms were all detected, and only carbendazim in Volvariella volvacea exceeded the standard, and the over-standard rate was 16.7 %. In a word, the rapid, sensitive and efficient CD-ELISA for quantifing carbendazim in mushrooms was established in the study.